Difference between revisions of "Part:BBa K3505021"

 
 
(7 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3505021 short</partinfo>
 
<partinfo>BBa_K3505021 short</partinfo>
  
long
+
Level 0 CDS
 +
[[File:T--Thessaly--djla.png|900px|thumb|none|<i><b>Fig.1:</b>DjlA</i>]]
 +
 
 +
 
  
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
DnaJ-like protein A (DjiA) is a membrane-bound DnaK co-chaperone that has antitoxic capacities for big protein expression in bacteria.[1]
 +
 +
 +
===Design Notes===
 +
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2,<bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning.
 +
The CDS has position B2-B5.
 +
[[File:T--Thessaly--GB-CCAT-GCTT.jpeg|800px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the GoldenBraid Grammar</i>]]
 +
 +
===Verification of cloning===
 +
[[File:T--Thessaly--d0.png|600px|thumb|none|<i><b>Fig.3:</b>Level 0 DjlA U4 C4 Digested with EcoRI, PstI Expected bands  2029, 894</i>]]
 +
 +
===Experimental Use and Experience===
 +
 +
This part is used in <bbpart>BBa_K3505039</bbpart>
 +
 +
===Source===
 +
Georgios Skretas [1]
  
<!-- -->
+
===Sequence and Features===
<span class='h3bb'>Sequence and Features</span>
+
 
<partinfo>BBa_K3505021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3505021 SequenceAndFeatures</partinfo>
  
  
<!-- Uncomment this to enable Functional Parameter display
+
===References===
===Functional Parameters===
+
*[1]Gialama, D., Delivoria, D., Michou, M., Giannakopoulou, A. and Skretas, G., 2017. Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. <em>Journal of Molecular Biology</em>, 429(12), pp.1800-1816.
<partinfo>BBa_K3505021 parameters</partinfo>
+
<!-- -->
+

Latest revision as of 00:12, 28 October 2020


DjlA- DnaJ-like protein A GB compatible with B2-B5

Level 0 CDS

Fig.1:DjlA


Usage and Biology

DnaJ-like protein A (DjiA) is a membrane-bound DnaK co-chaperone that has antitoxic capacities for big protein expression in bacteria.[1]


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2,BBa_K3505007 and has overhangs compatible for Golden Braid cloning. The CDS has position B2-B5.

Fig.2:The overhangs of this part in the GoldenBraid Grammar

Verification of cloning

Fig.3:Level 0 DjlA U4 C4 Digested with EcoRI, PstI Expected bands 2029, 894

Experimental Use and Experience

This part is used in BBa_K3505039

Source

Georgios Skretas [1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  • [1]Gialama, D., Delivoria, D., Michou, M., Giannakopoulou, A. and Skretas, G., 2017. Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. Journal of Molecular Biology, 429(12), pp.1800-1816.