Difference between revisions of "Part:BBa K3505021"

 
 
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<partinfo>BBa_K3505021 short</partinfo>
 
<partinfo>BBa_K3505021 short</partinfo>
  
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long
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Level 0 CDS
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[[File:T--Thessaly--djla.png|900px|thumb|none|<i><b>Fig.1:</b>DjlA</i>]]
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===Usage and Biology===
 
===Usage and Biology===
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DnaJ-like protein A (DjiA) is a membrane-bound DnaK co-chaperone that has antitoxic capacities for big protein expression in bacteria.[1]
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===Design Notes===
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2,<bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning.
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The CDS has position B2-B5.
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[[File:T--Thessaly--GB-CCAT-GCTT.jpeg|800px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the GoldenBraid Grammar</i>]]
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===Verification of cloning===
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[[File:T--Thessaly--d0.png|600px|thumb|none|<i><b>Fig.3:</b>Level 0 DjlA U4 C4 Digested with EcoRI, PstI Expected bands  2029, 894</i>]]
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===Experimental Use and Experience===
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This part is used in <bbpart>BBa_K3505039</bbpart>
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===Source===
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Georgios Skretas [1]
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3505021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3505021 SequenceAndFeatures</partinfo>
  
  
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===References===
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===Functional Parameters===
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*[1]Gialama, D., Delivoria, D., Michou, M., Giannakopoulou, A. and Skretas, G., 2017. Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. <em>Journal of Molecular Biology</em>, 429(12), pp.1800-1816.
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<partinfo>BBa_K3505021 parameters</partinfo>
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Latest revision as of 00:12, 28 October 2020


DjlA- DnaJ-like protein A GB compatible with B2-B5

Level 0 CDS

Fig.1:DjlA


Usage and Biology

DnaJ-like protein A (DjiA) is a membrane-bound DnaK co-chaperone that has antitoxic capacities for big protein expression in bacteria.[1]


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2,BBa_K3505007 and has overhangs compatible for Golden Braid cloning. The CDS has position B2-B5.

Fig.2:The overhangs of this part in the GoldenBraid Grammar

Verification of cloning

Fig.3:Level 0 DjlA U4 C4 Digested with EcoRI, PstI Expected bands 2029, 894

Experimental Use and Experience

This part is used in BBa_K3505039

Source

Georgios Skretas [1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  • [1]Gialama, D., Delivoria, D., Michou, M., Giannakopoulou, A. and Skretas, G., 2017. Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. Journal of Molecular Biology, 429(12), pp.1800-1816.