Difference between revisions of "Part:BBa K3657002"

Revision as of 00:12, 28 October 2020

RNA binding Pumilio Homology Domain (Uracil)

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.

This part is the Uracil binding Pumby Domain.

Combined with the other Pumby Domains:

Adenine:BBa_K3657001

Guanine:BBa_K3657002

Cytosine:BBa_K3657003

it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004, BBa_K3657005)

Usage and Biology

Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.

Sequence and Features