Difference between revisions of "Part:BBa K2765040"
 
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===<h1>FAFU-CHINA 2020 - Contribution</h1>===
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<p>Seung-pyo Hong et al[1] constructed an expression vector containing FBA1 in Yarrowia Lipolytica (pDMW212 & pDMW214), and measured its GUS Reportor activity and mRNA expression.
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</p>
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<ul>
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<li>https://2020.igem.org/wiki/images/d/dc/T--FAFU-CHINA--IMG28.png <br/>Figure 1. Histochemical and fluorometric GUS reporter assays to measure promoter activity. Fluorometric determination of GUS activity. GUSspecific activity was expressed as nM 4-methylumbelliferone released/min/mg protein.</li>
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__NOTOC__
 
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<partinfo>BBa_K2765040 short</partinfo>
 
  
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FBA1p is an constitutive promoter(the promoter of fructose-1,6-bisphosphate aldolase) from <i>Saccharomyces cerevisiae CEN.PK-1c</i>. In our project, we used it to turn on the expression of downstream fusion protein genes.
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<ul>
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<li>https://2020.igem.org/wiki/images/9/95/T--FAFU-CHINA--IMG29.png <br/>Figure 2. Relative quantities of GUS mRNA. The relative transcript levels were calculated using the GUS mRNA level from strains with TEF1::GUS as the reference. Assays were done in quadruplicate.</li>
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[[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1.png |center|400px|]]
 
  
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<h2>Reference</h2>
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===Usage and Biology===
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[1] Hong S P , Seip J , Walters-Pollak D , et al. Engineering Yarrowia lipolytica to express secretory invertase with strong FBA1IN promoter[J]. Yeast, 2012, 29(2).
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2765040 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2765040 parameters</partinfo>
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Latest revision as of 00:10, 28 October 2020

FAFU-CHINA 2020 - Contribution

Seung-pyo Hong et al[1] constructed an expression vector containing FBA1 in Yarrowia Lipolytica (pDMW212 & pDMW214), and measured its GUS Reportor activity and mRNA expression.

  • T--FAFU-CHINA--IMG28.png
    Figure 1. Histochemical and fluorometric GUS reporter assays to measure promoter activity. Fluorometric determination of GUS activity. GUSspecific activity was expressed as nM 4-methylumbelliferone released/min/mg protein.


  • T--FAFU-CHINA--IMG29.png
    Figure 2. Relative quantities of GUS mRNA. The relative transcript levels were calculated using the GUS mRNA level from strains with TEF1::GUS as the reference. Assays were done in quadruplicate.


Reference

[1] Hong S P , Seip J , Walters-Pollak D , et al. Engineering Yarrowia lipolytica to express secretory invertase with strong FBA1IN promoter[J]. Yeast, 2012, 29(2).