Difference between revisions of "Part:BBa K3657002"
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The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides. | The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides. | ||
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| − | <p> This part is the Uracil binding Pumby Domain. <p> | + | This part is the Uracil binding Pumby Domain. |
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Combined with the other Pumby Domains (<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a>, <a href="https://parts.igem.org/Part:BBa_K3657002">BBa_K3657002</a>, <a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a>) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: <a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>, <a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>) | Combined with the other Pumby Domains (<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a>, <a href="https://parts.igem.org/Part:BBa_K3657002">BBa_K3657002</a>, <a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a>) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: <a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>, <a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>) | ||
Revision as of 00:07, 28 October 2020
RNA binding Pumilio Homology Domain (Uracil)
The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.
This part is the Uracil binding Pumby Domain.
Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004, BBa_K3657005)
Usage and Biology
Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 70
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]