Difference between revisions of "Part:BBa K180000"
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The Ptac promoter sequence here is preceded by a coding region for lacIq; the function of this is to repress the promoter in its natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state. Ptac is also useful compared to its single-parent promoters trp pR and lac pR, as it has a stronger binding affinity than either of its parent promoters. | The Ptac promoter sequence here is preceded by a coding region for lacIq; the function of this is to repress the promoter in its natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state. Ptac is also useful compared to its single-parent promoters trp pR and lac pR, as it has a stronger binding affinity than either of its parent promoters. | ||
| − | ==Contribution== | + | ==Characterization== |
| + | ===Contribution=== | ||
This year LINKS_China improved the previous ptac promoter, part <partinfo>BBa_K180000</partinfo>, found in 2009 by reversing its lacI promoter, RBS, and terminator which will improve its yield of production. We also added three types of ribozymes, RiboJ, SccJ, and SarJ as an insulator to prevent the previous effect from the ptac promoter. | This year LINKS_China improved the previous ptac promoter, part <partinfo>BBa_K180000</partinfo>, found in 2009 by reversing its lacI promoter, RBS, and terminator which will improve its yield of production. We also added three types of ribozymes, RiboJ, SccJ, and SarJ as an insulator to prevent the previous effect from the ptac promoter. | ||
| − | |||
We first carried out an experiment in the culture medium with glucose as the source of carbon for the cultivation of bacteria in the M9 medium. In this experiment, we tested four ptac promoter, the old one, the new one, the one with RiboJ, and with SccJ. We convinced that all for ptac promoters are able to be activated when induced by IPTG, and as the concentration of IPTG increases, the expression of GFP genes promotes. Furthermore, the addition of two different types of ribozyme shows a prominent improvement of sensitivity towards the concentration of IPTG. At zero concentration of IPTG, all four promoter starts at a similar level, as the concentration of IPTG increases, the gradient of the graph increased dramatically. | We first carried out an experiment in the culture medium with glucose as the source of carbon for the cultivation of bacteria in the M9 medium. In this experiment, we tested four ptac promoter, the old one, the new one, the one with RiboJ, and with SccJ. We convinced that all for ptac promoters are able to be activated when induced by IPTG, and as the concentration of IPTG increases, the expression of GFP genes promotes. Furthermore, the addition of two different types of ribozyme shows a prominent improvement of sensitivity towards the concentration of IPTG. At zero concentration of IPTG, all four promoter starts at a similar level, as the concentration of IPTG increases, the gradient of the graph increased dramatically. | ||
Latest revision as of 00:04, 28 October 2020
Hybrid promoter (trp & lac regulated -- tac pR)
The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. The Ptac sequence has been extracted by PCR from the commercially available plasmid pMAL-pIII from New England Biolabs (N8101S), with the addition of the biobrick prefix and suffix sequences by a further PCR with specially designed primers.
[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The Ptac promoter sequence here is preceded by a coding region for lacIq; the function of this is to repress the promoter in its natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state. Ptac is also useful compared to its single-parent promoters trp pR and lac pR, as it has a stronger binding affinity than either of its parent promoters.
Characterization
Contribution
This year LINKS_China improved the previous ptac promoter, part BBa_K180000, found in 2009 by reversing its lacI promoter, RBS, and terminator which will improve its yield of production. We also added three types of ribozymes, RiboJ, SccJ, and SarJ as an insulator to prevent the previous effect from the ptac promoter.
We first carried out an experiment in the culture medium with glucose as the source of carbon for the cultivation of bacteria in the M9 medium. In this experiment, we tested four ptac promoter, the old one, the new one, the one with RiboJ, and with SccJ. We convinced that all for ptac promoters are able to be activated when induced by IPTG, and as the concentration of IPTG increases, the expression of GFP genes promotes. Furthermore, the addition of two different types of ribozyme shows a prominent improvement of sensitivity towards the concentration of IPTG. At zero concentration of IPTG, all four promoter starts at a similar level, as the concentration of IPTG increases, the gradient of the graph increased dramatically.
Afterward, according to our own medium, which utilizes glycerol as the only source of carbon, we attempted to simulate this situation for all five types of ptac promoter to investigate whether they can fit our experiments or not. Therefore, we substituted the medium to glycerol-M9 medium. The collected results imply that all five promoters can be activated obviously and the same as in glucose, promoters with ribozyme also obtain high sensitivity to the concentration of IPTG and dramatically increased.
These results confirmed the effect of our sensors under various types of carbon sources and maintain nearly no difference in practicability. We modified four new ptac promoter parts which are convinced to have higher sensitivity and adapt better to IPTG than the BBa_K180000 discovered in 2009.