Difference between revisions of "Part:BBa K3331000"
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<partinfo>BBa_K3331000 short</partinfo>
 
<partinfo>BBa_K3331000 short</partinfo>
  
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UTP-glucose-1-phosphate uridylyltransferase (GalU) carries out a key step in the generation of UDP-D-glucuronate.This part catalyzes the addition of UTP to &#945;-D-glucose 1-phosphate to yield UDP-D-glucose
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galU (ytdA) [Bacillus subtilis (strain 168)]
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Encodes UTP--glucose-1-phosphate uridylyltransferase that catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP in Bacillus subtilis.
  
 
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===Characterization===
 
===Characterization===
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We cloned and confirmed the target fragment by PCR. The length of the E.coli galU fragment is 981bp. The following results showed the successful result.
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We cloned and confirmed the target fragment by PCR. The length of our fragment is 951bp. The following results showed the successful result.
 
<div>[[File:T--XJTU-China--Bggel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>
 
<div>[[File:T--XJTU-China--Bggel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>
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<div>[[File:T--XJTU-China--Bgimprove02.jpeg |700px|thumb|center|]]</div>
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<div>[[File:T--XJTU-China--Bgimprove01.jpeg |700px|thumb|center|]]</div>
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the mRNA level of B.s GalU is ~2.4 fold higher than that of E.coli, indicating the significant improvement of enzyme expression and activity. However, due to the lower expression of another key enzyme PGM for EPS production in BE strain, the final EPS yield is lower than that of EE strain but still comparable.

Latest revision as of 23:48, 27 October 2020


B.S. galU

galU (ytdA) [Bacillus subtilis (strain 168)] Encodes UTP--glucose-1-phosphate uridylyltransferase that catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 951bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.
T--XJTU-China--Bgimprove02.jpeg
T--XJTU-China--Bgimprove01.jpeg

the mRNA level of B.s GalU is ~2.4 fold higher than that of E.coli, indicating the significant improvement of enzyme expression and activity. However, due to the lower expression of another key enzyme PGM for EPS production in BE strain, the final EPS yield is lower than that of EE strain but still comparable.