Difference between revisions of "Part:BBa K3657018:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | <html> | |
| − | + | The part was designed as a dimer from the original Broccoli aptamer with the use of <a href="https://github.com/igemsoftware2020/Heidelberg_2020/blob/main/CoNcoRDe/CoNcoRDe/rnasupport/scripts/design_simple.py">DesignSimple</a> in order to exclude the BsmbI cutting site and introduce the GUAGAG loop. | |
| − | + | <p>Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly <i>in vitro</i>.</p> | |
| + | </html> | ||
===Source=== | ===Source=== | ||
| − | + | Synthetic | |
===References=== | ===References=== | ||
| + | Wenjiao S, Strack R L, Nina S, et al. Plug-and-play fluorophores extend the spectral properties of Spinach.[J]. Journal of the American Chemical Society, 2014, 136(4):1198-1201. Filonov G S, Moon J D, Nina S, et al. Broccoli: Rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution.[J]. Journal of the American Chemical Society, 2014, 136. | ||
Latest revision as of 23:47, 27 October 2020
dBroccoli Aptamer with added GUAGAG RNA Loop
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 23
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was designed as a dimer from the original Broccoli aptamer with the use of DesignSimple in order to exclude the BsmbI cutting site and introduce the GUAGAG loop.
Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly in vitro.
Source
Synthetic
References
Wenjiao S, Strack R L, Nina S, et al. Plug-and-play fluorophores extend the spectral properties of Spinach.[J]. Journal of the American Chemical Society, 2014, 136(4):1198-1201. Filonov G S, Moon J D, Nina S, et al. Broccoli: Rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution.[J]. Journal of the American Chemical Society, 2014, 136.