Difference between revisions of "Part:BBa K3657000"
JuliaLanger (Talk | contribs) |
JuliaLanger (Talk | contribs) |
||
| Line 4: | Line 4: | ||
The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides. | The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides. | ||
| + | |||
This part is the Adenine binding Pumby Domain. | This part is the Adenine binding Pumby Domain. | ||
| + | |||
Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005) | Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005) | ||
Revision as of 23:40, 27 October 2020
RNA binding Pumilio Homology Domain (Adenine)
The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.
This part is the Adenine binding Pumby Domain.
Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005)
Usage and Biology
Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]