Difference between revisions of "Part:BBa K3440019"
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<partinfo>BBa_K3440019 short</partinfo>
 
<partinfo>BBa_K3440019 short</partinfo>
  
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Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxR(BBa_C0062)-DT(BBa_B0015)-Plux(BBa_R0062)-RBS(BBa_B0034)-mtrb(BBa_K3146001)-DT(BBa_B0015)
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Pconst(BBa_J23100) - RBS(BBa_B0034) - LuxR(BBa_C0062) - DT(BBa_B0015) - Plux(BBa_R0062) - RBS(BBa_B0034) - mtrb(BBa_K3146001) - DT(BBa_B0015)
  
 
===Usage and Biology===
 
===Usage and Biology===
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This part can be used to produce mtrb (gene responsible for electricity production in Shewanella oneidensis) in the presence of 3OC6-HSL.  
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This is an intermediate part of the oscillatory system which can be used to build our final modular biosensor BBa_K3440021. LuxR which is originally from Vibrio Fischeri (UniProtKB - P12746), is constitutively expressed under the  J23100 promoter. In the presence of 3OC6-HSL (a lactone produced by LuxI from Vibrio Fischeri, UniProtKB - P12747), the LuxR:3OC6-HSL complex can activate Plux, which controls production of MtrB. This oscillatory part will be present in Shewanella oneidensis MR-1 mtrb knockout.
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As this part is an intermediate in making the final oscillatory system it cannot be characterized individually unless added into BBa_K3440021.
  
 
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Latest revision as of 23:34, 27 October 2020


MtrB under Lux promoter

Pconst(BBa_J23100) - RBS(BBa_B0034) - LuxR(BBa_C0062) - DT(BBa_B0015) - Plux(BBa_R0062) - RBS(BBa_B0034) - mtrb(BBa_K3146001) - DT(BBa_B0015)

Usage and Biology

This is an intermediate part of the oscillatory system which can be used to build our final modular biosensor BBa_K3440021. LuxR which is originally from Vibrio Fischeri (UniProtKB - P12746), is constitutively expressed under the J23100 promoter. In the presence of 3OC6-HSL (a lactone produced by LuxI from Vibrio Fischeri, UniProtKB - P12747), the LuxR:3OC6-HSL complex can activate Plux, which controls production of MtrB. This oscillatory part will be present in Shewanella oneidensis MR-1 mtrb knockout. As this part is an intermediate in making the final oscillatory system it cannot be characterized individually unless added into BBa_K3440021.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985