Difference between revisions of "Part:BBa K3657000"

Revision as of 23:25, 27 October 2020

RNA binding Pumilio Homology Domain (Adenine)

Pumby Domains are engeneered from the Pumilio Homology Domain, which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.

This part is the Adenine binding Pumby Domain. Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005)

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3657000 short</partinfo>
-This part is a södfkasödfkjöaslkdjfölaksjdf
+Pumby Domains are engeneered from the Pumilio Homology Domain, which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
-<!-- Add more about the biology of this part here
+The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.
+This part is the Adenine binding Pumby Domain.
+Combined with the other Pumby Domains (BBa_K3657001, BBa_K3657002, BBa_K3657003) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004 and BBa_K3657005)
 ===Usage and Biology===