Difference between revisions of "Part:BBa K3506021"
 
(8 intermediate revisions by 4 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3506021 short</partinfo>
 
<partinfo>BBa_K3506021 short</partinfo>
  
−
U6 promoter is used to drive the transcription of homing guide RNA（hgRNA） in lineage tracing for eukaryotic systems. We used it to initiate the transcription of hgRNA(sgRNA). The U6 promoter has a clear transcription start point G. The 5′-TTTTT-3′ sequence located downstream of the promoter is used as a transcription termination signal, and the transcription product will be added at the 3′ end of 2 -4 oligomeric U.
+
<i>U6</i> promoter is used to initiate the transcription of homing guide RNA (hgRNA) in lineage tracing for eukaryotic systems. We used it to initiate the transcription of hgRNA(sgRNA). The <i>U6</i> promoter has a transcription start point G. The 5′-TTTTT-3′ sequence located downstream of the promoter is used as a transcription termination signal, and 2-4 oligomeric U will be added at the 3' end of the transcription product.
  
  
 
<b><font size="3">Biology and Usage</font></b>
 
<b><font size="3">Biology and Usage</font></b>
  
−
RNA polymerase III promoters uniquely transcribes small non-coding RNAs, including 5S rRNA , tRNAs, and other essential RNAs such as the U6 snRNA[1].U6 promoter is RNA polymerase III promoters and is a common part for eukaryotic expression systems. We use it to initiate the transcription of hgRNA used in CRISPR/Cas9 sysytem.You also can use the Pol Ⅲ promoter U6 to transcribe short hairpin RNA (shRNA,). The shRNA is cut by Dicer to become siRNA, which can interfere with the expression of target genes through the RNAi system.  
+
RNA polymerase III promoters initiate the transcription of small non-coding RNAs, including 5S rRNA, tRNAs, and other small RNAs such as the <i>U6</i> snRNA[1]. <i>U6</i> promoter is recognized by RNA polymerase III. We use it to initiate the transcription of hgRNA in CRISPR/Cas9 system. This promoter can also be used to transcribe short hairpin RNA (shRNA). The shRNA is recognized and digested by Dicers to generate siRNA, which can interfere with the expression of target genes through the RNAi system.
−
 
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 27: Line 26:
 
<b><font size="3">References</font></b>
 
<b><font size="3">References</font></b>
  
−
[1]Wang, Y., Wei, D., Zhu, X. et al. A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans. Sci Rep 6, 31145 (2016). https://doi.org/10.1038/srep31145
+
[1]Wang, Y., Wei, D., Zhu, X. et al. A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans. Sci Rep 6, 31145 (2016).
−
[2]Li Q, Wang W, Zhao N, Li P, Xin Y, Hu W. Identification and validation of a Schistosoma japonicum U6 promoter. Parasit Vectors. 2017;10(1):281. Published 2017 Jun 5. doi:10.1186/s13071-017-2207-4
+

Latest revision as of 23:20, 27 October 2020


U6 promoter

U6 promoter is used to initiate the transcription of homing guide RNA (hgRNA) in lineage tracing for eukaryotic systems. We used it to initiate the transcription of hgRNA(sgRNA). The U6 promoter has a transcription start point G. The 5′-TTTTT-3′ sequence located downstream of the promoter is used as a transcription termination signal, and 2-4 oligomeric U will be added at the 3' end of the transcription product.


Biology and Usage

RNA polymerase III promoters initiate the transcription of small non-coding RNAs, including 5S rRNA, tRNAs, and other small RNAs such as the U6 snRNA[1]. U6 promoter is recognized by RNA polymerase III. We use it to initiate the transcription of hgRNA in CRISPR/Cas9 system. This promoter can also be used to transcribe short hairpin RNA (shRNA). The shRNA is recognized and digested by Dicers to generate siRNA, which can interfere with the expression of target genes through the RNAi system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Wang, Y., Wei, D., Zhu, X. et al. A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans. Sci Rep 6, 31145 (2016).