Difference between revisions of "Part:BBa K3506021"

 
 
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<partinfo>BBa_K3506021 short</partinfo>
 
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U6 promoter is a strong driver of gRNA production, and was used to drive the expression of sgRNA in lineage tracing for eukaryotic expression systems. We used it to initiate the transcription of hgRNA(sgRNA).The U6 promoter has a clear transcription start point G. All promoter elements are located upstream of the transcription start point. The 5&#8242;-TTTTT-3&#8242; sequence located downstream of the promoter is used as a transcription termination signal, and the transcription product will be added at the 3&#8242; end of 2 -4 oligomeric U.
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<i>U6</i> promoter is used to initiate the transcription of homing guide RNA (hgRNA) in lineage tracing for eukaryotic systems. We used it to initiate the transcription of hgRNA(sgRNA). The <i>U6</i> promoter has a transcription start point G. The 5′-TTTTT-3′ sequence located downstream of the promoter is used as a transcription termination signal, and 2-4 oligomeric U will be added at the 3' end of the transcription product.
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<b><font size="3">Biology and Usage</font></b>
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RNA polymerase III promoters initiate the transcription of small non-coding RNAs, including 5S rRNA, tRNAs, and other small RNAs such as the <i>U6</i> snRNA[1]. <i>U6</i> promoter is recognized by RNA polymerase III. We use it to initiate the transcription of hgRNA in CRISPR/Cas9 system. This promoter can also be used to transcribe short hairpin RNA (shRNA). The shRNA is recognized and digested by Dicers to generate siRNA, which can interfere with the expression of target genes through the RNAi system.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K3506021 parameters</partinfo>
 
<partinfo>BBa_K3506021 parameters</partinfo>
 
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<b><font size="3">References</font></b>
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[1]Wang, Y., Wei, D., Zhu, X. et al. A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans. Sci Rep 6, 31145 (2016).

Latest revision as of 23:20, 27 October 2020


U6 promoter

U6 promoter is used to initiate the transcription of homing guide RNA (hgRNA) in lineage tracing for eukaryotic systems. We used it to initiate the transcription of hgRNA(sgRNA). The U6 promoter has a transcription start point G. The 5′-TTTTT-3′ sequence located downstream of the promoter is used as a transcription termination signal, and 2-4 oligomeric U will be added at the 3' end of the transcription product.


Biology and Usage

RNA polymerase III promoters initiate the transcription of small non-coding RNAs, including 5S rRNA, tRNAs, and other small RNAs such as the U6 snRNA[1]. U6 promoter is recognized by RNA polymerase III. We use it to initiate the transcription of hgRNA in CRISPR/Cas9 system. This promoter can also be used to transcribe short hairpin RNA (shRNA). The shRNA is recognized and digested by Dicers to generate siRNA, which can interfere with the expression of target genes through the RNAi system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Wang, Y., Wei, D., Zhu, X. et al. A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans. Sci Rep 6, 31145 (2016).