Difference between revisions of "Part:BBa K3407001:Design"
(2 intermediate revisions by one other user not shown) | |||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa | + | The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa. This part contains the activated toxin. The gene was codon-optimized for expression in <i>Escherichia coli</i> using the GenSmartTM Codon Optimization Tool. The forbidden restriction enzymes sites are the following: BioBrick forbidden restriction sites (EcoRI, XbaI, SpeI, PstI) and Type IIS forbidden restriction sites (BsaI, SapI and Bpil). |
− | + | ||
− | The | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
+ | Synthetically synthesised. | ||
− | |||
===References=== | ===References=== |
Latest revision as of 23:12, 27 October 2020
Cry7Ca1 toxin from Bacillus thuringiensis strain BTH-13
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 634
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1334
Illegal XhoI site found at 313 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa. This part contains the activated toxin. The gene was codon-optimized for expression in Escherichia coli using the GenSmartTM Codon Optimization Tool. The forbidden restriction enzymes sites are the following: BioBrick forbidden restriction sites (EcoRI, XbaI, SpeI, PstI) and Type IIS forbidden restriction sites (BsaI, SapI and Bpil).
Source
Synthetically synthesised.
References