Difference between revisions of "Part:BBa K3610020:Design"

 
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===Design Notes===
 
===Design Notes===
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Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standard
+
Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standard.
−
 
+
  
 +
Split was made at amino acids 158 and 159 of the full length mCherry.
  
 
===Source===
 
===Source===
  
−
The sequence was taken of Part:BBa_K1093016 from the iGEM registry and then codon optimized with SnapGene.
+
Provided by the Cyril Zipfel Lab, Switzerland
  
 
===References===
 
===References===
 +
Fan, Jin-Yu; Cui, Zong-Qiang; Wei, Hong-Ping; Zhang, Zhi-Ping; Zhou, Ya-Feng; Wang, Yun-Peng; Zhang, Xian-En (2008): Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. In: Biochemical and Biophysical Research Communications 367 (1), S. 47–53. DOI: 10.1016/j.bbrc.2007.12.101.

Latest revision as of 23:10, 27 October 2020


mCherry N-terminal - codon optimized for S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standard.

Split was made at amino acids 158 and 159 of the full length mCherry.

Source

Provided by the Cyril Zipfel Lab, Switzerland

References

Fan, Jin-Yu; Cui, Zong-Qiang; Wei, Hong-Ping; Zhang, Zhi-Ping; Zhou, Ya-Feng; Wang, Yun-Peng; Zhang, Xian-En (2008): Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. In: Biochemical and Biophysical Research Communications 367 (1), S. 47–53. DOI: 10.1016/j.bbrc.2007.12.101.