Difference between revisions of "Part:BBa K3610021:Design"

(→‎Design Notes)
(→‎Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The sequence was taken from the registry and then adapted for expression in C. reinhardtii
+
Provided by the Cyril Zipfel Lab, Switzerland
  
 
===References===
 
===References===

Revision as of 23:10, 27 October 2020


mCherry C-terminal - codon optimized for S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.

The split was made at amino acids 158 and 159 from the full length mCherry.

Source

Provided by the Cyril Zipfel Lab, Switzerland

References