Difference between revisions of "Part:BBa K133015"

Latest revision as of 05:06, 30 October 2008

CF215-mCherry-RGD-Histop

CF215 is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215). In combination with CF213 it forms a TLR5 activating protein.

mCherry is a red fluorescent protein that can be used for localisation studies.

Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 571
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 128
Illegal BamHI site found at 639
Illegal BamHI site found at 1603
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1638

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K133015 short</partinfo>
-CF215 is the C-terminal portion of chimeric fusion protein; it contains the  C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215). In combination with CF213 it forms a TLR5 activating protein.
+CF215 is the C-terminal portion of chimeric fusion protein; it contains the  C-terminal segment of the hypervariable region of <i>H. pylori</i> flagellin FlaA from 215 AA + 99 AA of C-terminal portion from <i>E. coli</i> flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215). In combination with CF213 it forms a TLR5 activating protein.
 mCherry is a red fluorescent protein that can be used for localisation studies.
+Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.