Difference between revisions of "Part:BBa K3657026"

 
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This part is a fusion protein consisting of SoxS activator (<a href="https://parts.igem.org/Part:BBa_K3657024">BBa_K3657024</a>) and MS2 capsid protein (<a href="https://parts.igem.org/Part:BBa_K3218006">BBa_K3218006</a>).
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===Usage and Biology===
 
===Usage and Biology===
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<p>MS2 capsid protein binds specifically to the MS2 RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3113006">BBa_K3113006</a>). SoxS is a powerful activator in <i>E. coli</i>.</p>
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<p>This construct was used together with a RNA&middot;DNA-DNA triple helix containing an MS2 hairpin (<a href="https://parts.igem.org/Part:BBa_K3657025">BBa_K3657025</a>). Use of these two parts together allows to attract the SoxS activator to the specific location on a DNA. More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a>.</p>
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<p>Due to the COVID19 pandemic we were not able to get any empirical evidence about the performance of this part.</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3657026 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3657026 SequenceAndFeatures</partinfo>

Latest revision as of 22:59, 27 October 2020


MS2 Coat Protein + SoxS Trancription Activator
This part is a fusion protein consisting of SoxS activator (BBa_K3657024) and MS2 capsid protein (BBa_K3218006).

Usage and Biology

MS2 capsid protein binds specifically to the MS2 RNA hairpin (BBa_K3113006). SoxS is a powerful activator in E. coli.

This construct was used together with a RNA·DNA-DNA triple helix containing an MS2 hairpin (BBa_K3657025). Use of these two parts together allows to attract the SoxS activator to the specific location on a DNA. More information available here: iGEM Heidelberg 2020.

Due to the COVID19 pandemic we were not able to get any empirical evidence about the performance of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]