Difference between revisions of "Part:BBa K3380780"

 
 
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<partinfo>BBa_K3380780 short</partinfo>
 
<partinfo>BBa_K3380780 short</partinfo>
  
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Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the native Cupriavidus Metallidurans CH34 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
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Taken from the work of Millacura, Valenzuela-Ortega and French 2019.
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===Usage and Biology===
 
===Usage and Biology===
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Can be used in a cell-free biosensor
  
 
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Latest revision as of 22:54, 27 October 2020


TXO czcN regulated construct under BBa_K3380109 promoter expressing iSpinach

Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the native Cupriavidus Metallidurans CH34 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.

Taken from the work of Millacura, Valenzuela-Ortega and French 2019.


Usage and Biology

Can be used in a cell-free biosensor

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]