Difference between revisions of "Part:BBa K133008"

Latest revision as of 04:57, 30 October 2008

CF213-UB33

This part is composed of CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

UB33 is a peptide derived from the catalytic site of beta subunit of H. pylori urease. Urease is an important pathogenicity factor for H. pylori since it enables the bacteria to breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 718
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 301
1000
COMPATIBLE WITH RFC[1000]

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 This part is composed of
-CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal  portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
+CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal  portion of <i>E. coli</i> flagellin + N-terminal segment of the hypervariable region of <i>H. pylori</i> flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
-UB33 is a peptide derived from the catalytic site of beta subunit of H. pylori urease. Urease is an important pathogenicity factor for H. pylori since it enables the bacteria to breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.
+UB33 is a peptide derived from the catalytic site of beta subunit of <i>H. pylori</i> urease. Urease is an important pathogenicity factor for <i>H. pylori</i> since it enables the bacteria to breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.
 <!-- Add more about the biology of this part here