Difference between revisions of "Part:BBa K133007"

 
 
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<partinfo>BBa_K133007 short</partinfo>
 
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This part is composed of:
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CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of <i>E. coli</i> flagellin + N-terminal segment of the hypervariable region of <i>H. pylori flagellin</i> FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
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CF215 – the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of <i>H. pylori</i> flagellin FlaA from 215 AA + 99 AA of C-terminal portion from <i>E. coliy</i> flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
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We also designed synthetic multiepitope, comprising the <i>H. pylori</i> urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Multiepitope is a synthetic gene orderd from GeneArt.
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Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:56, 30 October 2008

CF213-multiHP-CF215-RGD-Histop

This part is composed of:

CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

CF215 – the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coliy flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

We also designed synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Multiepitope is a synthetic gene orderd from GeneArt.

Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1746
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1303
    Illegal BamHI site found at 1814
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2097