Difference between revisions of "Part:BBa K142203:Design"

(Design Notes)
(Source)
 
Line 10: Line 10:
 
===Source===
 
===Source===
  
source
+
The construct was synthesized by Geneart.
  
 
===References===
 
===References===

Latest revision as of 04:14, 30 October 2008

T4 ligase with RBS (BBa_B0030) and double terminator (BBa_B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 353


Design Notes

Sequence was optimised to remove Biobrick restriction sites.

Source

The construct was synthesized by Geneart.

References