Difference between revisions of "Part:BBa K1608002"

 
 
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<partinfo>BBa_K1608002 short</partinfo>
 
<partinfo>BBa_K1608002 short</partinfo>
  
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In order to express SR ([https://parts.igem.org/Part:BBa_K1608000 BBa_K1608000]) and SRPK ([https://parts.igem.org/Part:BBa_K1608001 BBa_K1608001]) gene more conveniently, we made two composite biobricks with promoter, RBS and a fusion tag.
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<h2>Contribution</h2>
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Team: PuiChing_Macau 2020<br>
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Summary: We have compared the original SR without adhesion domains and our engineered SR with adhesion domains, by the fire retardancy test. As shown in figure 1, the protein with adhesion domain has a great improvement on its fire retardancy.
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<img src="https://2020.igem.org/wiki/images/9/9c/T--Puiching_Macau--bedsheet_chart.png" width="600">
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<div class="legend">Figure 1. Vertical Burning Test using bedsheet
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<h4>Conclusion</h4>
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<p>Overall, the proteins with adhesion retain a higher fire retardancy than the protein without adhesion through the fire retardancy test. Therefore, the genetic fusion between SR protein and adhesion domain would generate a more stable fire retardant protein.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 22:14, 27 October 2020

GST-SR/pSB1C3

In order to express SR (BBa_K1608000) and SRPK (BBa_K1608001) gene more conveniently, we made two composite biobricks with promoter, RBS and a fusion tag.

Contribution

Team: PuiChing_Macau 2020
Summary: We have compared the original SR without adhesion domains and our engineered SR with adhesion domains, by the fire retardancy test. As shown in figure 1, the protein with adhesion domain has a great improvement on its fire retardancy.

Figure 1. Vertical Burning Test using bedsheet

Conclusion

Overall, the proteins with adhesion retain a higher fire retardancy than the protein without adhesion through the fire retardancy test. Therefore, the genetic fusion between SR protein and adhesion domain would generate a more stable fire retardant protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1338
    Illegal BglII site found at 1407
    Illegal BglII site found at 1524
    Illegal BamHI site found at 798
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 210