Difference between revisions of "Part:BBa K3610011"

Latest revision as of 22:12, 27 October 2020

CORE ectodomain - codon optimized for C. reinhardtii

This part is a modification of Part:BBa_K3610010. It is codon optimized for expression in C. reinhardtii.

Biology

The cold shock protein receptor (CORE) is a plant pattern recognition receptor (PRR) and as such activates host innate immunity through detection of pathogen-associated molecular patterns (PAMPs). CORE is a leucine-rich repeat receptor-like kinase with 22 LRRs, there additionally is a 6 amino acid insert at LRR 11. It consists of an extracellular domain that perceives an epitope, csp22, from the highly conserved nucleic acid binding motif RNP-1 of bacterial cold-shock proteins (CSPs), which are highly abundant proteins found in the cytosol of bacteria. Further domains are a single pass transmembrane domain and an intracellular kinase domain (The sequence encoding the kinase domain is not in this part). Interaction of CORE with brassinosteroid-associated kinase (BAK)1 is necessary for inducing an immune response in the plant. The dimerization of CORE and BAK1 depends on the csp22, the ligand of CORE. The function of CORE in S. lycopersicum has been confirmed by expressing the receptor in A. thaliana, which made the plant responsive to csp22, a PAMP that is otherwise not perceived by PRRs from A. thaliana.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 918
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1764

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3610011 short</partinfo>
-This part is a modification of [[Part:BBa_K3610010]]. It is codon optimized for expression in C. reinhardtii.
+This part is a modification of [[Part:BBa_K3610010]]. It is codon optimized for expression in <i>C. reinhardtii</i>.
+===Biology===
+The cold shock protein receptor (CORE) is a plant pattern recognition receptor (PRR) and as such activates host innate immunity through detection of pathogen-associated molecular patterns (PAMPs). CORE is a leucine-rich repeat receptor-like kinase with 22 LRRs, there additionally is a 6 amino acid insert at LRR 11. It consists of an extracellular domain that perceives an epitope, csp22, from the highly conserved nucleic acid binding motif RNP-1 of bacterial cold-shock proteins (CSPs), which are highly abundant proteins found in the cytosol of bacteria. Further domains are a single pass transmembrane domain and an intracellular kinase domain (The sequence encoding the kinase domain is not in this part). Interaction of CORE with brassinosteroid-associated kinase (BAK)1 is necessary for inducing an immune response in the plant. The dimerization of CORE and BAK1 depends on the csp22, the ligand of CORE. The function of CORE in <i>S. lycopersicum</i> has been confirmed by expressing the receptor in <i>A. thaliana</i>, which made the plant responsive to csp22, a PAMP that is otherwise not perceived by PRRs from A. thaliana.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
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