Difference between revisions of "Part:BBa K3657016"
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===Usage and Biology===
 
===Usage and Biology===
 
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Broccoli is an aptamer that forms a stable secondary structure and binds the fluorogen DFHBI, which results in green fluorescence. The absence of BsmbI site allows cloning of this part with <a href="http://2018.igem.org/Team:Marburg/Part_Collection">Marburg Collection protocols</a>.
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Broccoli is an aptamer that forms a stable secondary structure and binds the fluorogen DFHBI, which results in green fluorescence. The absence of BsmbI site allows cloning of this part with <a href="http://2018.igem.org/Team:Marburg/Part_Collection">Marburg Collection system</a>.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 21:48, 27 October 2020


Broccoli Aptamer without BsmbI site

This part is a modification of the original Broccoli aptamer. It was re-engineered to exclude the BsmbI cutting site.


Usage and Biology

Broccoli is an aptamer that forms a stable secondary structure and binds the fluorogen DFHBI, which results in green fluorescence. The absence of BsmbI site allows cloning of this part with Marburg Collection system. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]