Difference between revisions of "Part:BBa K3454037"
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<partinfo>BBa_K3454037 short</partinfo>
 
<partinfo>BBa_K3454037 short</partinfo>
  
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This part is the template for AsCas12a extraction and purfication for reaction system. It consists of three parts, which are AsCas12a coding sequence, MBP tag and His tag.
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This part is the template for AsCas12a extraction and purfication for reaction system.
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===Characterization===
 
===Characterization===
 
====Results====
 
====Results====

Revision as of 21:43, 27 October 2020


AsCas12a

This part is the template for AsCas12a extraction and purfication for reaction system.

Characterization

Results

We induced AsCas12a expression by IPTG and purified it by gravity-flow column with Ni-NTA Sefinose(TM) Resin. CBB(Coomassie brilliant blue) is used to characterize protein purification as shown in Figure 1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 886
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3855
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
    Illegal NgoMIV site found at 3399
    Illegal AgeI site found at 2296
    Illegal AgeI site found at 2767
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1039
    Illegal BsaI.rc site found at 2701
    Illegal SapI.rc site found at 289