Difference between revisions of "Part:BBa K121010"
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<partinfo>BBa_K121010 short</partinfo>
 
<partinfo>BBa_K121010 short</partinfo>
  
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This part is use as a positive control of Plac GFP reporter.  
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Promoter (tetR replessed) + RBS + GFP + TT  on pSB6 ([[Part:BBa_I739201]]).
  
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=== Pressure response ===
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We measured pressure response of BBa_K121010 in BBa_I739003 in E. Coli strain JM109.
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[[Image:Pressure_response_of_tet_promoter.png|thumb|500px|center|figure. Pressure response of Ptet. Ptet activity increased about 3-fold under 30 MPa ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here]]]
 
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===Usage and Biology===
 
===Usage and Biology===
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Latest revision as of 03:55, 30 October 2008

Ptet GFP on pSB6

Promoter (tetR replessed) + RBS + GFP + TT on pSB6 (Part:BBa_I739201).

Pressure response

We measured pressure response of BBa_K121010 in BBa_I739003 in E. Coli strain JM109.

figure. Pressure response of Ptet. Ptet activity increased about 3-fold under 30 MPa ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724