Difference between revisions of "Part:BBa K121012"
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<partinfo>BBa_K121012 short</partinfo>
 
<partinfo>BBa_K121012 short</partinfo>
  
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pressure and IPTG inducible Plac GFP reporter
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IPTG inducible Plac (lacI regulated) + RBS + GFP + TT on pSB6 ([[Part:BBa_I739201]]).
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Usage and Biology===
 
===Usage and Biology===
  
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'''Identify Hill coefficient by IPTG induction'''  
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==== Identify Hill coefficient by IPTG induction ====
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We identified hill coefficient of Plac_GFP in BBa_I739003 in ''E. Coli'' strain JM109.
  
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Using iGEM Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in ''E. Coli'' strain JM109.
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[[Image:Transfer_Function_K121012.png|thumb|500px|center|figure. Transfer Function of BBa_K121012. The data points represent the mean of experimental measurements. The solid green curve was calculated by fitting a Hill function to the experimental measurements. The characteristics of Plac were calculated by fitting Hill function to the plots. We obtained the value of the hill coefficient. the value is 2.2. ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here]]]
  
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[[Image:Transfer Function K121012.png]]
 
  
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The hill coefficient is 2.15.
 
  
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'''Pressure induction'''
 
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Latest revision as of 03:54, 30 October 2008

Plac GFP on pSB6

IPTG inducible Plac (lacI regulated) + RBS + GFP + TT on pSB6 (Part:BBa_I739201).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 267
    Illegal BamHI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 954

Usage and Biology

Identify Hill coefficient by IPTG induction

We identified hill coefficient of Plac_GFP in BBa_I739003 in E. Coli strain JM109.

figure. Transfer Function of BBa_K121012. The data points represent the mean of experimental measurements. The solid green curve was calculated by fitting a Hill function to the experimental measurements. The characteristics of Plac were calculated by fitting Hill function to the plots. We obtained the value of the hill coefficient. the value is 2.2. ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here]