Difference between revisions of "Part:BBa K3452602"

Latest revision as of 21:20, 27 October 2020

MntH

It expressed it on Bacillus thuringiensis using it to take up cadmium ions. Bacillus subtilis also belongs to the NRAMP family, which is a proton-coupled metal ion transporter.We use it to transport Cd ion into synechocystis.

Contribution

Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary: We have successfully expressed MntH in E. coli DH5α(Figure1 a). At the same time, When cadmium concentrations rise, E.coli expressing MntH is more likely to die（Figure1. d), which is supposed to be the transport of cadmium into cells leading to cell death. But it showes a very strong ability to absorb cadmium.（Figure1. e）

Figure1: (a) WB proves the successful expression of our protein, (b) Growth curves of chelated protein (SmtA, hMT-1A, TMCd1)-expressed E. coli, (c) Growth curves of APX2-expressed E. coli, (d) Cadmium uptake curve of (MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli, (e) Growth curves of ( MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 166
Illegal NgoMIV site found at 508
Illegal NgoMIV site found at 937
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 Group: iGEM20_CSU_CHINA (2020-10-26)<br>
 Author: Hao Zhou<br>
-Summary:  We have successfully expressed MntH in E. coli DH5α(Figure1 a). The OD600 of E.coli expressing MntH ,which represents that the concentration of E. coli, will increase lower than that without expression（Figure1. b). It may be due to the transport of cadmium ions into cells leading to cell death. At the same time, When cadmium concentrations rise, it is more likely to die（Figure1. d), But it showes a very strong ability to absorb cadmium.（Figure1. e）<br>
+Summary:  We have successfully expressed MntH in E. coli DH5α(Figure1 a).  At the same time, When cadmium concentrations rise, E.coli expressing MntH is more likely to die（Figure1. d), which is supposed to be the transport of cadmium into cells leading to cell death. But it showes a very strong ability to absorb cadmium.（Figure1. e）<br>
 <center>https://2020.igem.org/wiki/images/thumb/a/ae/T--CSU_CHINA--figure-engineering_success.png/800px-T--CSU_CHINA--figure-engineering_success.png</center><br>
 <center>Figure1: (a) WB proves the successful expression of our protein, (b) Growth curves of chelated protein (SmtA, hMT-1A, TMCd1)-expressed E. coli, (c) Growth curves of APX2-expressed E. coli, (d) Cadmium uptake curve of (MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli, (e) Growth curves of ( MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli.</center><br>