Difference between revisions of "Part:BBa K3452020"

Latest revision as of 21:01, 27 October 2020

APX2

Arabidopsis thaliana ascorbate peroxidase 2 (APX2) The expression level of APX2 in Arabidopsis cells increases in response to cadmium stress. Overexpression of APX2 significantly decreases oxidative stress in cadmium-stressed cells. Molecular mechanism studies have shown that it can effectively protect the balance of the photosynthetic systems by eliminating the disorder of the electron transport chain in photosynthesis under cadmium stress.

Contribution

Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary: We have successfully expressed APX2 in E. coli DH5α(Figure1 a). The OD600 of E.coli expressed by APX2 represents that the concentration of E. coli , will increase faster than that without expression（Figure1. c). At the same time, it can maintain a high strain concentration at different cadmium concentrations（Figure1. d)

Figure1: (a) WB proves the successful expression of our protein, (b) Growth curves of chelated protein (SmtA, hMT-1A, TMCd1)-expressed E. coli, (c) Growth curves of APX2-expressed E. coli, (d) Cadmium uptake curve of (MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli, (e) Growth curves of ( MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 Group: iGEM20_CSU_CHINA (2020-10-26)<br>
 Author: Hao Zhou<br>
-Summary:  We have successfully expressed APX2 in E. coli DH5α(Figure1 a). The OD600 of E.coli expressed by APX2 represents that the concentration of bacteria, will increase faster than that without expression（Figure1. c). At the same time, it can maintain a high strain concentration at different cadmium concentrations（Figure1. d). and indirectly shows a certain cadmium absorption ability.（Figure1. e）<br>
+Summary:  We have successfully expressed APX2 in E. coli DH5α(Figure1 a). The OD600 of E.coli expressed by APX2 represents that the concentration of E. coli , will increase faster than that without expression（Figure1. c). At the same time, it can maintain a high strain concentration at different cadmium concentrations（Figure1. d)<br>
 <center>https://2020.igem.org/wiki/images/thumb/a/ae/T--CSU_CHINA--figure-engineering_success.png/800px-T--CSU_CHINA--figure-engineering_success.png</center><br>
 <center>Figure1: (a) WB proves the successful expression of our protein, (b) Growth curves of chelated protein (SmtA, hMT-1A, TMCd1)-expressed E. coli, (c) Growth curves of APX2-expressed E. coli, (d) Cadmium uptake curve of (MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli, (e) Growth curves of ( MntH / hMT-1A / MntH & TMCd1 & APX2)-expressed E. coli.</center><br>