Difference between revisions of "Part:BBa K3505027"

Latest revision as of 20:58, 27 October 2020

PfliC:RBS-lac repressor:terminator

LacI BBa_K3505003uder control of a SCFAs inducible promoter pFLliC BBa_K2924016.

Fig.1:pFliC-LacI-Terminator

Usage and Biology

This Trancriscription Unit (TU) is activated form SCFAs and more specifically from Butyrate. Exressing the LacI protein for the inhibition of an other TU that is lac regulated.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in BBa_K3505008 and has overhangs compatible for GoldenBraid cloning.

Verification of cloning

Fig.2:(U=Uncut C=Cut) Restriction Enzyme with BamHI, HpaI Expected bands in bp 2847 + 1348, 4195

Experimental Use and Experinece

This part is used in BBa_K3505036

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 LacI <bbpart>BBa_K3505003</bbpart>uder control of a SCFAs inducible promoter pFLliC <bbpart>BBa_K2924016</bbpart>.
-[[File:T--Thessaly--pflic-laci-PHOT.png|600px|thumb|none|<i><b>Fig.1:</b>pFliC-LacI-Terminator</i>]]
+[[File:T--Thessaly--pflic-lacI-PHOT.png|600px|thumb|none|<i><b>Fig.1:</b>pFliC-LacI-Terminator</i>]]
 ===Usage and Biology===
@@ Line 13: / Line 13: @@
 ===Design Notes===
-The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in <bbpart>BBa_K3505008</bbpart> and has overhangs compatible for Golden Braid cloning.
+The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in <bbpart>BBa_K3505008</bbpart> and has overhangs compatible for GoldenBraid cloning.
 ===Verification of cloning===