Difference between revisions of "Part:BBa K3656305"
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| − | This part is the coding sequence of the Pyrococcus horikoshii OT3 | + | This part is the coding sequence of the Pyrococcus horikoshii OT3 Carbonic Anhydrase Wild Type(OT3-CA-WT). It is a γ-class carbonic anhydrase，with high thermal stability(50 to over 125℃) but low catalytic activity. In our project, it is the original sequence. Based on this sequence, We have processed and modified it in order to improve its catalytic activity. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 20:41, 27 October 2020
OT3-Carbonic Anhydrase-Wild Type
This part is the coding sequence of the Pyrococcus horikoshii OT3 Carbonic Anhydrase Wild Type(OT3-CA-WT). It is a γ-class carbonic anhydrase，with high thermal stability(50 to over 125℃) but low catalytic activity. In our project, it is the original sequence. Based on this sequence, We have processed and modified it in order to improve its catalytic activity. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 470
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]