Difference between revisions of "Part:BBa K3396014"
 
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===Usage and Biology===
 
===Usage and Biology===
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This part was designed by us to add one of protein pair with high affinity conveniently. Two restriction sites of BsaI were added to the 3’ end of XTEN Linker, through which, the 3’ untranslated region of any gene can be inserted using Golden-Gate Assembly, with no worry about the multiple illegal sites contained in it. Furthermore, NanoLuc gene can be replaced with any protein in the same way.
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This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two BsaI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly.
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<html>
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<p><img src="https://static.igem.org/mediawiki/parts/c/cf/T--NUDT_CHINA--Repla-2.png" alt="" width="500" /></p>
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</html>
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Figure 1. Schematic representation of the Replaceable-1.
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===Sequence and Features===
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 20:22, 27 October 2020


Repleceable-2

This is the XTEN gene flanked with specific restriction sites that could be recognized by BsaI.

Usage and Biology

This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two BsaI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly.


Figure 1. Schematic representation of the Replaceable-1.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 55
    Illegal BsaI.rc site found at 1