Difference between revisions of "Part:BBa K3630029"

Latest revision as of 20:08, 27 October 2020

Enhanced ferritin expression system

As our BREAC is a microorganism that could tip the balance of the aquarium ecosystem, its removal is another necessary problem that we have to address. Aquariums usually have a filter to clean the water. However, it cannot guarantee the removal of our bacteria. Hence, our design has evolved to include to have the third feature of the project, that is, the magnetization system. When a magnetic field is applied, E. coli can be magnetized and stick on the magnet and it could be cleared.

Constitutively promotes ferritin expression. The ferritin has been linked to iBox-PAK4 which forms crystal structures and enhances the bacteria's sensitivity to magnetic force.

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Figure.Magnetization expression construction

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3630029 short</partinfo>
-Constitutively promote ferritin to accumulate Fe ion and make bacteria become positive change. The ferritin that we make has already been mutated and combine with iBox-PAK4. iBox- PAK4 can crystalization the protein and enhance the accumulation of Fe ion to increase the magnetic ability.
+As our BREAC is a microorganism that could tip the balance of the aquarium ecosystem, its removal is another necessary problem that we have to address. Aquariums usually have a filter to clean the water. However, it cannot guarantee the removal of our bacteria. Hence, our design has evolved to include to have the third feature of the project, that is, the magnetization system. When a magnetic field is applied, E. coli can be magnetized and stick on the magnet and it could be cleared.
+Constitutively promotes ferritin expression. The ferritin has been linked to iBox-PAK4 which forms crystal structures and enhances the bacteria's sensitivity to magnetic force.
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