Difference between revisions of "Part:BBa K3482014"
 
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===Biology and Usage===
 
===Biology and Usage===
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The IM2 molecule is the immunity protein of the Colicin E2 endonuclease. The IM2 protein is here under un pTet promoter and can therefore be activated by aTc.
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The IM2 molecule is the immunity protein of the Colicin E2 endonuclease. The IM2 protein is here under a pTet promoter and can therefore be activated by aTc.  
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This part is used to create a kill-switch in combination with the part <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3482004"> BBa_K3482004 </a></html>.
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This part is used to create a kill switch in combination with part <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3482004"> BBa_K3482004 </a></html>.
  
 
===Characterization===
 
===Characterization===

Latest revision as of 19:43, 27 October 2020


aTc-inducible IM2 antitoxin

IM2 antitoxin under a pTet promoter

Biology and Usage

The IM2 molecule is the immunity protein of the Colicin E2 endonuclease. The IM2 protein is here under a pTet promoter and can therefore be activated by aTc.

This part is used to create a kill switch in combination with part BBa_K3482004 .

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482004 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 242
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]