Difference between revisions of "Part:BBa K143062"

Latest revision as of 02:48, 30 October 2008

LacI repressor protein - Terminator

LacI transcriptional repressor protein (BBa_K143033) coupled to the double terminator (BBa_B0015).

The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis. LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.

Please note. This part has picked up a mutation, which changes an amino acid residue from a Serine to a Leucine but does not cause a frame shift. Given the size of the protein, this should not adversley afect its function to a significant degree.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K143062 short</partinfo>
-LacI transcriptional repressor protein (<bbpart>BBa_K143033</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>.
-The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with ''B. subtilis''.
+LacI transcriptional repressor protein (<bbpart>BBa_K143033</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).
+The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with ''B. subtilis''. LacI can be used in conjunction with the lac operon promoter (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a '''Polymerases per second''' (PoPS) output.
-LacI can be used in conjunction with the lac operon promoter (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a '''Polymerases per second''' (PoPS) output.
 The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.
 The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.
+Please note. This part has picked up a mutation, which changes an amino acid residue from a Serine to a Leucine but does not cause a frame shift. Given the size of the protein, this should not adversley afect its function to a significant degree.
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 22: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 <partinfo>BBa_K143062 SequenceAndFeatures</partinfo>