Difference between revisions of "Part:BBa K3365009:Design"
Little ghost (Talk | contribs) (→References) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | lure3 is | + | The lure3 sequence is contained in the reverse primer. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | The lure3 sequence is predicted by our off-target predictor software built in 2019 and ligated to the promoter of the L-arabinose operon of E. coli (pBAD) by PCR. | |
===References=== | ===References=== | ||
| + | [1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30. | ||
Latest revision as of 19:23, 27 October 2020
Lure3 sequence downstream of pBAD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 74
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 56
Design Notes
The lure3 sequence is contained in the reverse primer.
Source
The lure3 sequence is predicted by our off-target predictor software built in 2019 and ligated to the promoter of the L-arabinose operon of E. coli (pBAD) by PCR.
References
[1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30.