Difference between revisions of "Part:BBa K3482011"

Latest revision as of 19:22, 27 October 2020

pTac promoter

The pTac promoter sequence here is preceded by a binding region (operator) for the repressor LacI; the binding of LacI prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to LacI and releases it from its operator.

We use this pTac promoter in our kill switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3482011 short</partinfo>
-The Ptac promoter sequence here is preceeded by a coding region for lacI; the function of this is to repress the promoter in it's natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state.
+The pTac promoter sequence here is preceded by a binding region (operator) for the repressor <i>LacI</i>; the binding of <i>LacI</i> prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to <i>LacI</i> and releases it from its operator.
-We use this pTac promoter in our kill-switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.
+We use this pTac promoter in our kill switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3482011 SequenceAndFeatures</partinfo>