Difference between revisions of "Part:BBa K3482011"

 
 
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<partinfo>BBa_K3482011 short</partinfo>
 
<partinfo>BBa_K3482011 short</partinfo>
  
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The pTac promoter sequence here is preceded by a binding region (operator) for the repressor <i>LacI</i>; the binding of <i>LacI</i> prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to <i>LacI</i> and releases it from its operator.
  
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===Usage and Biology===
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We use this pTac promoter in our kill switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3482011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3482011 SequenceAndFeatures</partinfo>

Latest revision as of 19:22, 27 October 2020


pTac promoter

The pTac promoter sequence here is preceded by a binding region (operator) for the repressor LacI; the binding of LacI prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to LacI and releases it from its operator.


We use this pTac promoter in our kill switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]