Difference between revisions of "Part:BBa K3600003"
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The GSH1 gene encodes gamma-glutamylcysteine synthetase.<html><a href="#Wu1994"><sup>1</sup></a></html> | The GSH1 gene encodes gamma-glutamylcysteine synthetase.<html><a href="#Wu1994"><sup>1</sup></a></html> | ||
| − | The GSH1 promoter is regulated by the transcription factor Yap1p. The binding site for Yap1p, in the promoter GSH1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a>. Using the yeast toolkit for modular assembly, the GSH1 promoter plasmid was used as a part of type II. | + | The GSH1 promoter is regulated by the transcription factor Yap1p. The binding site for Yap1p, in the promoter GSH1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a></html>. Using the yeast toolkit for modular assembly, the GSH1 promoter plasmid was used as a part of type II. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3600003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3600003 SequenceAndFeatures</partinfo> | ||
| − | < | + | <--! |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3600003 parameters</partinfo> | <partinfo>BBa_K3600003 parameters</partinfo> | ||
Latest revision as of 19:21, 27 October 2020
GSH1 promoter part plasmid
GSH1 promoter part plasmid was made in order to create a part plasmid which contains the GSH1 promoter and is compatible with the Yeast Toolkit. To create a promoter part plasmid compatible with the yeast toolkit, at first, we designed a gBlock consisting of the 500bp promoter region, that was retrieved from the yeast genome database, and two linking sequences which were added to the ends of the promoter.These linking sequences contained a BsaI and a BsmBI cutting site each to make Golden Gate assembly possible.Then, the gBlock was inserted into the part plasmid entry vector (pYTK001) through a BsmBI assembly.
Usage and Biology
The GSH1 gene encodes gamma-glutamylcysteine synthetase.1 The GSH1 promoter is regulated by the transcription factor Yap1p. The binding site for Yap1p, in the promoter GSH1, is TTACTAA.1. Using the yeast toolkit for modular assembly, the GSH1 promoter plasmid was used as a part of type II.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 975
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 563
Illegal BsaI site found at 972
Illegal BsaI.rc site found at 1084
<--!
Functional Parameters