Difference between revisions of "Part:BBa K3332087"
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We use pTrc-2 derivative promoter to express ECFP to check the function of pTrc-2 derivative promoter
 
We use pTrc-2 derivative promoter to express ECFP to check the function of pTrc-2 derivative promoter
  
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<!-- Add more about the biology of this part here
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With this part, the pTrc-2 derivative promoter can be tested by observing the fluorescence intensity/OD.
 
===Usage and Biology===
 
===Usage and Biology===
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This part can be used to test that if the pTrc-2 derivative promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 derivative promoter.
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<html>
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    <figure>
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        <img src="https://2020.igem.org/wiki/images/0/0f/T--XMU-China--XMU-China_2020-pTrc-2_derivative_B0034_ecfp_B0015.png" width="60%" style="float:center">
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        <figcaption>
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        <p style="font-size:1rem">
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        </p>
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        </figcaption>
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    </figure>
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</html>
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'''Fig 1. '''pTrc-2 derivative_RBS(B0034)_ECFP(E0020)_terminator(B0015)
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===Characterization===
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The agarose gel electrophoresis images are shown as below:
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[[File:T--XMU-CHINA--BBa K3332087.png|none|500px|caption]]
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'''Fig 2.''' pTrc-2 derivative_E0420_pSB1C3[BBa_K3332087] digested by ''Xba'' I and ''Pst'' I (about 1018 bp)
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===Reference===
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[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. &apos;Deadman&apos; and &apos;Passcode&apos; microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979
  
 
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Revision as of 18:43, 27 October 2020


pTrc-2 derivative-RBS-ECFP-terminator

We use pTrc-2 derivative promoter to express ECFP to check the function of pTrc-2 derivative promoter

With this part, the pTrc-2 derivative promoter can be tested by observing the fluorescence intensity/OD.

Usage and Biology

This part can be used to test that if the pTrc-2 derivative promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 derivative promoter.

Fig 1. pTrc-2 derivative_RBS(B0034)_ECFP(E0020)_terminator(B0015)

Characterization

The agarose gel electrophoresis images are shown as below:

caption

Fig 2. pTrc-2 derivative_E0420_pSB1C3[BBa_K3332087] digested by Xba I and Pst I (about 1018 bp)

Reference

[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. 'Deadman' and 'Passcode' microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]