Difference between revisions of "Part:BBa K3482035"

 
 
(3 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3482035 short</partinfo>
 
<partinfo>BBa_K3482035 short</partinfo>
  
−
Azurin with pelB tag for secretion and 8His tag for protein purification of secreted azurin.
+
Azurin fused with a 8xHis tag and with a pelB secretion signal peptide for protein expression and purification
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.
 +
  
 
<!-- -->
 
<!-- -->

Latest revision as of 18:41, 27 October 2020


azurin with pelB secretion and 8His purification tag

Azurin fused with a 8xHis tag and with a pelB secretion signal peptide for protein expression and purification

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]