Difference between revisions of "Part:BBa K3600001"
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<partinfo>BBa_K3600001 short</partinfo> | <partinfo>BBa_K3600001 short</partinfo> | ||
| − | + | TRX2 promoter part plasmid was made in order to create a part plasmid which contains the TRX2 promoter and is compatible with the Yeast Toolkit. To create a promoter part plasmid compatible with the yeast toolkit, at first, we designed a gBlock consisting of the 500bp promoter region, that was retrieved from the yeast genome database, and two linking sequences which were added to the ends of the promoter.These linking sequences contained a BsaI and a BsmBI cutting site each to make Golden Gate assembly possible.Then, the gBlock was inserted into the part plasmid entry vector (pYTK001) through a BsmBI assembly. | |
===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | TRX2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.<html><a href="#Kuge1994"><sup>1</sup></a></html> | ||
| + | The TRX2 promoter is regulated by the transcription factor Yap1p. The binding site for Yap1p, in the promoter TRX2, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a></html> | ||
| + | Using the yeast toolkit for modular assembly, <html><a href="#Lee2015"><sup>2</sup></a></html> the HSP12 promoter plasmid was used as a part of type II. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3600001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3600001 SequenceAndFeatures</partinfo> | ||
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<HTML><a name=Kuge1994 style="color:black"> | <HTML><a name=Kuge1994 style="color:black"> | ||
[1] Kuge, S. & Jones, N. YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. The EMBO Journal 13, 655–664 (1994). | [1] Kuge, S. & Jones, N. YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. The EMBO Journal 13, 655–664 (1994). | ||
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| + | <HTML><a name=Martínez-Pastor1996 style="color:black"> | ||
| + | [2] Martínez-Pastor, M. T. et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). The EMBO Journal 15, 2227–2235 (1996). | ||
</a></html> | </a></html> | ||
Latest revision as of 18:26, 27 October 2020
TRX2 promoter part plasmid
TRX2 promoter part plasmid was made in order to create a part plasmid which contains the TRX2 promoter and is compatible with the Yeast Toolkit. To create a promoter part plasmid compatible with the yeast toolkit, at first, we designed a gBlock consisting of the 500bp promoter region, that was retrieved from the yeast genome database, and two linking sequences which were added to the ends of the promoter.These linking sequences contained a BsaI and a BsmBI cutting site each to make Golden Gate assembly possible.Then, the gBlock was inserted into the part plasmid entry vector (pYTK001) through a BsmBI assembly.
Usage and Biology
TRX2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.1 The TRX2 promoter is regulated by the transcription factor Yap1p. The binding site for Yap1p, in the promoter TRX2, is TTACTAA.1 Using the yeast toolkit for modular assembly, 2 the HSP12 promoter plasmid was used as a part of type II.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 568
Illegal BsaI.rc site found at 1089