Difference between revisions of "Part:BBa K3380750"
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| − | + | Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the copAB transcription factor. Upon addition of Cu, the promoter allows the ''E.coli'' sigma 70 (and native ''Cupriavidus Metallidurans'') polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added. | |
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Latest revision as of 17:37, 27 October 2020
TXO copAB regulated construct under BBa_K3380108 promoter expressing iSpinach
Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the copAB transcription factor. Upon addition of Cu, the promoter allows the E.coli sigma 70 (and native Cupriavidus Metallidurans) polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]