Difference between revisions of "Part:BBa K3380660"
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<partinfo>BBa_K3380660 short</partinfo> | <partinfo>BBa_K3380660 short</partinfo> | ||
| − | + | Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the ZntR transcription factor. Upon addition of Zinc, the promoter allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Can be used in cell-free systems as a biosensor. | ||
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Latest revision as of 17:30, 27 October 2020
TXO ZntR regulated construct under BBa_K3380105 promoter expressing iSpinach
Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the ZntR transcription factor. Upon addition of Zinc, the promoter allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
Usage and Biology
Can be used in cell-free systems as a biosensor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]