Difference between revisions of "Part:BBa K3468051"
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<partinfo>BBa_K3468051 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3468051 SequenceAndFeatures</partinfo> | ||
| + | For the G139N mutation, the substitution of Asparagine for Glycine in mutation G139N conferred new hydrogen bonds with the carboxyl of A135 | ||
| + | [[File:8051.png|400px|]] | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3468051 parameters</partinfo> | <partinfo>BBa_K3468051 parameters</partinfo> | ||
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Latest revision as of 17:09, 27 October 2020
PETase G139N
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from G to N at 139 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
For the G139N mutation, the substitution of Asparagine for Glycine in mutation G139N conferred new hydrogen bonds with the carboxyl of A135
