Difference between revisions of "Part:BBa K3408002"

Revision as of 17:07, 27 October 2020

Trigger RNA(of toehold)

Trigger RNA is a short RNA sequence that can bind to the switch RNA to initiate translation of downstream gene.

In our gene circuit, we need high stability and long half-life of trigger RNA. Based on literature, we found that 5' hairpin could improve stability of mRNA. By imitating this design, we added 5’ hairpin to trigger RNA to meet our need.

In our project we would use trigger RNA to release the expression of mazF when switch-mazF is expressed in wormcast. So our engineered bacteria could commit suicide.

Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 110
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_k3408002 short</partinfo>
-It is the part of toehold switch which functions as the key to relieve switch RNA and activate downstream circuit.
+Trigger RNA is a short RNA sequence that can bind to the switch RNA to initiate translation of downstream gene.
-We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
+In our gene circuit, we need high stability and long half-life of trigger RNA. Based on literature, we found that 5' hairpin could improve stability of mRNA. By imitating this design, we added 5’ hairpin to trigger RNA to meet our need.
-In our project we would use the trigger RNA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacteria could commit suicide.
+In our project we would use trigger RNA to release the expression of <i>mazF</i> when switch-mazF is expressed in wormcast. So our engineered bacteria could commit suicide.