Difference between revisions of "Part:BBa K3332039"

Revision as of 16:25, 27 October 2020

tetR

The tetR protein is able to repress pLtetO-1 promoter in the absence of ATc.

Usage and Biology

The tetR protein is used to inhibit pLtetO-1. It is part of the circuit designed to prevent engineered bacteria in the detection instrument from escaping.

Fig 1. Kill switch of the detection part

In this circuit, LacI can repress pTrc-2 promoter and pTrc-2 derivative promoter ，while tetR can repress pLtetO-1 promoter. When ATc exits, it combines with tetR, so that pLtetO-1 promoter can’t be repressed. Then LacI which is controlled by pLtetO-1 can repress the pTrc-2 promoter and pTrc-2 derivative promoter. As a result, mf-lon and MazF can’t be expressed.

As a kind of bacterial toxin, the expression of MazF often lead to the death of bacteria. So there comes the conclusion that as long as the engineered E.coli are cultured in the environment with ATc, it won’t be killed by the mazF, but when the E.coli escape from our detection instrument, the effect can be reversed. That is to say, the E.coli will be killed by MazF. In the same way, we can see that in the presence of IPTG, MazF can be expressed and kill theE.coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. 'Deadman' and 'Passcode' microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3332039 short</partinfo>
-The tetR protein is able to repress pLtetO-1 promoter in the absence of aTc.
+The tetR protein is able to repress pLtetO-1 promoter in the absence of ATc.
 ===Usage and Biology===
-The tetR protein is used to inhibit pLtetO-1 from expressing LacI. It is part of the circut designed to prevent engineered bacteria in the detection instrument from escaping.
+The tetR protein is used to inhibit pLtetO-1. It is part of the circuit designed to prevent engineered bacteria in the detection instrument from escaping.
-<table><tr><th>[[File:T--XMU-CHINA--circuit--circuit.png|thumb|600px|Fig.1 Circuit.]]</th><th></table>
+<table><tr><th>[[File:T--XMU-CHINA--circuit--circuit.png|thumb|600px|'''Fig 1.''' Kill switch of the detection part]]</th><th></table>
-In this circuit, LacI can repress pTrc-2 promoter and pTrc-2 derivative promoter，while tetR can repress pLtetO-1 promoter. When ATc exits, it can combine tetR, so that pLtetO-1 promoter can’t be repressed. Then LacI which is controlled by pLtetO-1 can repress pTrc-2 promoter and pTrc-2 derivative promoter. As a result, mf-lon and MazF can’t be expressed.
+In this circuit, LacI can repress pTrc-2 promoter and pTrc-2 derivative promoter ，while tetR can repress pLtetO-1 promoter. When ATc exits, it combines with tetR, so that pLtetO-1 promoter can’t be repressed. Then LacI which is controlled by pLtetO-1 can repress the pTrc-2 promoter and pTrc-2 derivative promoter. As a result, mf-lon and MazF can’t be expressed.
-As a kind of bacterial toxin, MazF can cause the bacteria death. So there comes the conclusion that as long as the engineered E.coli are cultured in the environment with ATc, it won’t be killed by MazF, but when the E.coli escape from our testing instrument, the effect can be reversed, that is to say, the E.coli will be killed by MazF. In the same way, we can conclude that in the presence of IPTG, MazF can be expressed to cause bacterial death.
+As a kind of bacterial toxin, the expression of MazF often lead to the death of bacteria. So there comes the conclusion that as long as the engineered ''E.coli'' are cultured in the environment with ATc, it won’t be killed by the mazF, but when the ''E.coli'' escape from our detection instrument, the effect can be reversed. That is to say, the ''E.coli'' will be killed by MazF. In the same way, we can see that in the presence of IPTG, MazF can be expressed and kill the''E.coli''.
 ===Sequence and Features===