Difference between revisions of "Part:BBa K3599034"

 
 
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<partinfo>BBa_K3599034 short</partinfo>
 
<partinfo>BBa_K3599034 short</partinfo>
  
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a modified green fluorescent protein, we used its sequence as a reporting gene to measure the intensity of the expression.
 
  
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<!-- Add more about the biology of this part here
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===Overview===
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===Usage and Biology===
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A reporter module of XylS sensor device. This module is <b>an improvement version of [[Part:BBa_K1031941|BBa_K1031941]]</b> (<b><i>Important!!!</i></b>), which is designed by [http://2013.igem.org/Team:Peking Peking iGEM2013].
  
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K3599034 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3599034 SequenceAndFeatures</partinfo>
  
  
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<!-- Uncomment this to enable Functional Parameter display
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===Design & Experience===
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===Functional Parameters===
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To characterize the property of the device itself, the original aromatic compond m-Toluic acid (mTa) was used as inducer. According to the experment results provided by [http://2013.igem.org/Team:Peking Peking iGEM2013], the mTa could cause the largest dynamic range and the most sensity of the Pm-eGFP sensor.
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<partinfo>BBa_K3599034 parameters</partinfo>
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====The fluorescence of the sensor induced by small molecules====
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Through the corresponding small molecule sensing experiment, except Dmpr sensor, all our sensors have great induction effects on its corresponding aromatic small molecules. Among which NahR-sfGFP was the best! It is 63 times of the negative control. <b>And here we can see that Xyls-sfGFP is 18 times of the Xyls-eGFP ([[Part:BBa_K1031941|BBa_K1031941]])</b>. And what surprises us is that Paax sensor also has a good response effect to small molecules, which has not been detected before.<br>
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[[File:T--TPR China--Sensor1.png|600px|thumb|center|The fluorescence of the sensor induced by small molecules]]<br>
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[[File:T--TPR China--Sensor2.png|600px|thumb|center|The fluorescence of the NahR sensor induced by small molecules]]<br>
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====The fluorescence of the sensor induced by PAN====
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And here is the fluorescence of different sensors induced by PAN.This data shows the same result that NahR-sfGFP responded best.
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[[File:T--TPR China--Sensor3.jpg|600px|thumb|center|The fluorescence of the sensor induced by PAN]]<br>

Latest revision as of 15:57, 27 October 2020


Pm-sfGFP


Overview

A reporter module of XylS sensor device. This module is an improvement version of BBa_K1031941 (Important!!!), which is designed by [http://2013.igem.org/Team:Peking Peking iGEM2013].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 188


Design & Experience

To characterize the property of the device itself, the original aromatic compond m-Toluic acid (mTa) was used as inducer. According to the experment results provided by [http://2013.igem.org/Team:Peking Peking iGEM2013], the mTa could cause the largest dynamic range and the most sensity of the Pm-eGFP sensor.

The fluorescence of the sensor induced by small molecules

Through the corresponding small molecule sensing experiment, except Dmpr sensor, all our sensors have great induction effects on its corresponding aromatic small molecules. Among which NahR-sfGFP was the best! It is 63 times of the negative control. And here we can see that Xyls-sfGFP is 18 times of the Xyls-eGFP (BBa_K1031941). And what surprises us is that Paax sensor also has a good response effect to small molecules, which has not been detected before.

The fluorescence of the sensor induced by small molecules

The fluorescence of the NahR sensor induced by small molecules

The fluorescence of the sensor induced by PAN

And here is the fluorescence of different sensors induced by PAN.This data shows the same result that NahR-sfGFP responded best.

The fluorescence of the sensor induced by PAN