Difference between revisions of "Part:BBa K1362424"

Latest revision as of 15:48, 27 October 2020

BsaI restriction site for RFC[105] cloning

This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible post-translational modifications.

Information added by Team IISER-Pune-India 2020

Team: IISER-Pune-India 2020

Author: Avadhoot Jadhav

Summary

We aimed to use this part for circularization of the cyclotide protein. For using this we found that it had 7 nucleotide sequences that made it incompatible for Gibson's assembly/Restriction free cloning. The 7 length sequence makes it out of frame with other components of the composite structure. So without disturbing the restriction site we designed a similar part that overcomes this problem: BBa_K3582021.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1362424 short</partinfo>
-This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
+This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible post-translational modifications.
+===Information added by Team IISER-Pune-India 2020===
+<b>Team</b>: IISER-Pune-India 2020
+<b>Author</b>: Avadhoot Jadhav
+<b>Summary</b>
+We aimed to use this part for circularization of the cyclotide protein. For using this we found that it had 7 nucleotide sequences that made it incompatible for Gibson's assembly/Restriction free cloning. The 7 length sequence makes it out of frame with other components of the composite structure. So without disturbing the restriction site we designed a similar part that overcomes this problem: BBa_K3582021.
 <!-- Add more about the biology of this part here