Difference between revisions of "Part:BBa K1931014"
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This part codes for the bacteriocin Microcin E492 with MBP fusion protein | This part codes for the bacteriocin Microcin E492 with MBP fusion protein | ||
| − | [ https://2020.igem.org/Team:Ionis_Paris Team Ionis_Paris 2020] contributed to this part by adding information learnt from the literature, a 3D structure of the part and existing experiments to characterize the part. The original composite part was not correctly annotated, so we performed a blast to add the features of each sequence composing the part. We did not have the time to test this part in the lab but it could be very useful in the case of our project, BacTail which goal is to fight against antimicrobial resistance with a antimicrobial peptide producing bacterium [visit our wiki https://2020.igem.org/Team:Ionis_Paris/Contribution]. | + | <h2> <b> Ionis_Paris 2020 Documentation </b> </h2> |
| + | Group: Ionis_Paris 2020 | ||
| + | <br> | ||
| + | Author: Giulia Criseo | ||
| + | <br> | ||
| + | Summary: [ https://2020.igem.org/Team:Ionis_Paris Team Ionis_Paris 2020] contributed to this part by adding information learnt from the literature, a 3D structure of the part and existing experiments to characterize the part. The original composite part was not correctly annotated, so we performed a blast to add the features of each sequence composing the part. We did not have the time to test this part in the lab but it could be very useful in the case of our project, BacTail which goal is to fight against antimicrobial resistance with a antimicrobial peptide producing bacterium [visit our wiki https://2020.igem.org/Team:Ionis_Paris/Contribution]. | ||
| + | |||
| + | === Usage=== | ||
| + | Bacteriocins are antimicrobial proteins naturally synthesized by bacteria to inhibit the growth of closely related species1. They are produced by the strains ‘’Eubacteria’’ and ‘’Archaebacteria’’ and are excreted in order to specifically recognize and destroy certain bacteria2. Bacteriocins can kill using different mechanisms, such as the formation of membrane pores. They are widely used as preservatives in the food industry or as antibiotics in the health industry3. | ||
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Revision as of 14:56, 27 October 2020
T7-RBS-Microcin E492-MBP-Terminator
This part codes for the bacteriocin Microcin E492 with MBP fusion protein
Ionis_Paris 2020 Documentation
Group: Ionis_Paris 2020
Author: Giulia Criseo
Summary: [ https://2020.igem.org/Team:Ionis_Paris Team Ionis_Paris 2020] contributed to this part by adding information learnt from the literature, a 3D structure of the part and existing experiments to characterize the part. The original composite part was not correctly annotated, so we performed a blast to add the features of each sequence composing the part. We did not have the time to test this part in the lab but it could be very useful in the case of our project, BacTail which goal is to fight against antimicrobial resistance with a antimicrobial peptide producing bacterium [visit our wiki https://2020.igem.org/Team:Ionis_Paris/Contribution].
Usage
Bacteriocins are antimicrobial proteins naturally synthesized by bacteria to inhibit the growth of closely related species1. They are produced by the strains ‘’Eubacteria’’ and ‘’Archaebacteria’’ and are excreted in order to specifically recognize and destroy certain bacteria2. Bacteriocins can kill using different mechanisms, such as the formation of membrane pores. They are widely used as preservatives in the food industry or as antibiotics in the health industry3.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]